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(higher outside than inside) The unequal charge distribution is discussed in terms of volts and is called the membrane potential In reality, multiple ions contribute to the net membrane potential The direction that something will move spontaneously is determined by the free energy ( G) for that movement, which is going to depend on direction The G for moving from out to in will be of the opposite sign of the G for moving from in to out When you re trying to decide which way something might move spontaneously (where G is negative), you have to assume a direction, calculate the G for that direction, and look at the sign If it s negative (favorable), things will move in the direction you assumed If it s positive, things will move in the opposite direction For any molecule distributed across a membrane at equilibrium, the overall G will be the sum Gion Gconc Thus, at equilibrium zFV 136 log (Cout /Cin) If Cout /Cin is 10, then V will be 136(kcal/mol)(1)/(1 23 kcal/V/mol) 136/23 006 V (or 60 mV) The conclusion is that a ratio of 10:1 for a positive ion outside the cell will create a voltage difference of 60mV (negative side) By convention, the potential difference is defined with respect to the inside a more negative inside will have a negative membrane potential while a more positive inside will have a positive membrane potential There are lots of questions that can be asked about your understanding of membrane potential One common question is to give you a membrane potential, a few concentrations of ions, and ask which way will things move spontaneously Which way will an ion move It will move so that the G is 0 (favorable) Let s do an example Assume that the membrane potential of the cell is 60 mV (inside) This is near the potential that the cell normally maintains and denotes that there are more negative ions inside the cell than outside Let s also assume that the concentration of Na is 20 mM outside and 100 mM inside Now, if we were to punch a hole in the membrane that would allow Na to move (a channel), which way would it move The signs of G are dictated by what you call inside and outside, so let s try to figure out the magnitude of G and determine the sign of G by intuition Because the cell is negative inside, the membrane potential would tend to cause the Na to move from outside to inside ( likes ) If we assume that the Na would move from outside to inside, this would correspond to a Gion (1)23(006) 138 kcal/mol The sign would be negative because moving inside would be good (remember likes ) Now you have to consider the concentration gradient Since the Na is higher inside than outside, the concentration gradient would make it harder to move the Na from outside to inside (what we assumed originally) The Gconc would be positive and equal to 136*log(20/100) 136*log(20/100) 136( 07) 095 kcal/mol The easiest thing to do here is to forget whether outside or inside is on top of the.

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Yet another theory concerning the origin and anatomy of the quasars suggests that they are points in space through which new matter is entering from some other space-time continuum Such objects, in the vernacular of the cosmologist, are called white holes As matter bursts into our spacetime continuum, having been pulled from another Universe by overwhelming gravitational forces, the flash of radiant energy would outshine any typical galaxy Direct evidence to support this theory is lacking, but it is one of the most fascinating in all cosmology It implies that there exist other universes with space-time singularities connecting them with ours

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ratio of concentrations If you make a mistake here, all it will do is change the sign of G, not the magnitude of the number So be sure that you determine if each G makes sense If we had made that mistake here, the Gconc we calculated would be 095 kcal/mol instead of 095 kcal/mol But this must be wrong we know that with a higher concentration inside, the Na would like to move down its concentration gradient Since we re calculating the G for moving from outside to inside (we assumed that), then the Gconc must be positive We ve just found that the Gion is 138 kcal/mol for moving Na from outside to inside and that Gconc is 095 kca/mol Since the total G is negative ( 138 095), the Na will move in the direction we assumed (outside to inside) Even though the concentration gradient is opposing the movement, the strength of the membrane potential is sufficient to cause the ion to move up its concentration gradient This requires energy which in this case must come from maintaining the membrane potential You ve just seen how a pump works

Once you return to the Filters tab, you can also set the usage for the filter The default value for Usage is Always, which means that the filter criteria are always included when the query subject is executed That makes sense most of the time You would always want a security filter to be in use, or one that eliminates unwanted data But there are two other Usage settings The first is Optional, which only applies to filters with prompts A prompt is a filter with a test value consisting of a prompt variable surrounded by question marks, like this:

= 1 Also, since the system is nominally level, Therefore,

In recent years, the idea that black holes exist at the centers of many, if not most, spiral galaxies has been gaining acceptance This is so in part because of an interesting twist in the formula for the Schwarzchild radius as a function of mass

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