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teriophage-encoded peptidoglycan hydrolases that are produced in phage-infected bacterial cells to degrade peptidoglycan, thereby ensuring the release of progeny virions (lysins are described in more detail in 1) Below are characterized lysins derived from staphylococcal phages, including MV-L, LysK, PlyTW, Ply187, and S aureus 11 phage lysin MV-L is a lysin encoded by S aureus phage MR11 (Rashel et al 2007) This enzyme contains two enzymatic domains, the rst of which is homologous to the cysteine, histidine-dependent aminohydrolases/ peptidases (CHAP) domain (endopeptidase) and the other to the Ami-2 domain (amidase) In vitro, MV-L rapidly lysed all 13 S aureus strains tested, including nine MRSA strains and a lysostaphin-resistant MSSA strain (MRSA and MSSA strains were lysed with comparable ef cacy) While MV-L was also capable of lysing two VRSA strains and seven VISA strains under growing conditions, the other 11 VISA strains could only be lysed under nongrowing conditions.

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function Therefore, by using the ag bit, it is not dif cult to locate a particular set of bits that activate a given arbitrary Boolean function This is essential for those dif cult problems in which a really involved Boolean function is known but for which inputs that satisfy the function (to make it true) are not obvious Possible Applications By applying a sequence of from and to operations, a Boolean operation can be implemented on a subset of bits in each word (in parallel) of the memory system For example, consider a single word with N = 7 for the purposes of explaining a self-analyzing memory word Initialize to a7 a6 a5 a4, a3 a2 a1 a0 = 1010, 1110; assume that we want the following Boolean function of six inputs, AND {a7 (a6)ua5 (a4)ua3 a2 a1}; we want to indicate the result by toggling a0.

In this rst section we will focus on tangible and abstract entities, those existing architectures, software, and physical components that either have been or are currently common in the enterprise technology infrastructure.

Importantly, a synergistic effect against a VISA strain was noted for the combination of MV-L and vancomycin Interestingly, unlike lysostaphin, MV-L did not display activity against S epidermidis The inability of MV-L to kill S epidermidis may prove advantageous because this species can inhibit the growth of S aureus In a murine model of nasal colonization, one topical dose of lysin administered to mice 60 h after intranasal MRSA inoculation completely cleared bacteria in one of nine mice and substantially reduced the colonization in the remaining eight animals In a murine model of MRSA bacteremia, injection of a single dose of MV-L 60 min after a lethal dose of MRSA reduced the mortality rate to 40%, whereas administration of the enzyme either simultaneously with or 30 min after MRSA rescued 100% of the mice Another anti-staphylococcal lytic enzyme is lysin encoded by S aureus 11 bacteriophage (Navarre et al.

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