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This equation does not describe the binding site(s) or he binding af nity To incorporate these parameters and estimate the extent of binding, double-reciprocal plots of 1/[TP] versus 1/[T ] may be used to test the speci city of binding The 1/[TP] term can also be interpreted as moles of albumin per moles of toxicant The slope of the straight line equals 1/nKa and the intercept of this line with the x-axis equals Ka Regression lines passing through the origin imply in nite binding, and the validity of calculating an af nity constant under these circumstances is questionable Figure 610 illustrates one such case with four pesticides, and the insert illustrates the low-af nity, unsaturable nature of binding in this example.

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The two classes of toxicant-protein interactions encountered may be de ned as (1) speci c, high af nity, low capacity, and (2) nonspeci c, low af nity, high capacity The term high af nity implies an af nity constant (Kbinding ) of the order of 108 M 1 , whereas low af nity implies concentrations of 104 M 1 Nonspeci c, low-af nity binding is probably most characteristic of nonpolar compounds, although most cases are not as extreme as that shown in Figure 610 An alternative and well-accepted treatment for binding studies is the Scatchard equation especially in situations of high-af nity binding: = nk[T ] , 1 + k[T ].

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