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1 ECF buffers react essentially instantaneously to an acid or base load. The intracellular buffers, greater in buffering capacity than those in the ECF, consist of intracellular proteins and various phosphates that buffer acid and base loads on a time scale of hours. The enormous amount of protein in skeletal muscle is a key component, along with hemoglobin in red blood cells. Buffering by bone is also significant (and rather complicated) on an even slower time scale (see 10). 2 The actual reaction involves combining CO2 with a hydroxyl ion already bound to the enzyme, resulting in the immediate formation of bicarbonate (HCO3 ). As the bicarbonate dissociates from the enzyme, a water molecule binds to it. The water is then split into a hydrogen ion that dissociates from the enzyme and a hydroxyl ion that stays behind on the enzyme. The end result is that a CO2 molecule and a water molecule have been converted to a hydrogen ion and a bicarbonate, the same as if they had gone through the slower uncatalyzed reaction of first forming a carbonic acid molecule.

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This writer s impressive accomplishments warrant a lengthy letter. Note how the writer skillfully breaks the first page in the middle of a compelling statement to encourage the reader to turn to the second page.

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In Stage 2, the increase in scale improves production, spreads fixed costs and earns profits. However, profits are still slim because pricing becomes extremely competitive. And, as always, strategy is key to gaining or maintaining a winning position. Companies must determine which market segments to stake out, and not all of them are equally rewarding. Some segments may be unattractive because they require disproportionate investments; others may be desirable because they offer a foothold into a new or newly accessible market. As a result, companies must determine how much investment is required or, more specifically, estimate the return on capital that will be provided in their desired segments. Similarly, while companies must be quick to consolidate and grow, they must be careful to avoid taking on more than they can handle

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Make sure that the external MIDI device is connected to your computer using the port labeled External MIDI, MIDI OUT, or MPU-401. Make sure there are speakers or headphones connected to the external MIDI device. Check to see that those speakers or headphones are turned on and that the volume is turned up.

It s a good sign. It s a better sign if the seller accepts your original offer. But a counter means the seller is willing to dicker with you. After a seller counters at a signi cantly lower price (but not as low as you ve originally offered), you ve really got only three alternatives:

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Part 2:

control of sodium and water excretion / 105

Caution Running an Internet-accessible Web server on your network requires at least 50 Kbps of upload bandwidth or more per simultaneous visitor depending on the size of images or files. This can quickly swamp your Internet connection, which is why most small businesses pay for Web hosting.

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