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This is because the response of the system is never flat within the spectral band pass, and thus spectrally varying object views of different integrated radiances can produce the same output In other words, uneven spectral responses are linked with radiometric errors The SpectRx Spectroradiometer represents an improvement over non-spectral radiometers First, the addition of many spectral channels contributes to the narrowing of each channel, thus potentially making each of them more uniform Another advantage is that it may be possible to restore distorted signals If spectral channels are located in a contiguous manner, spectrally side-by-side, an over-evaluation of a given channel is accompanied by the complementary under-evaluation in neighboring channels This means that the correct radiance of a feature in the spectrum can be evaluated by spectrally integrating over all channels that responded to the signal Spectroscopically, this is a known phenomenon.

When the ShowSave statement is encountered, a dialog box resembling that shown in Fig. 9.5 will appear. The user may then use this dialog box to save a file within the currently active folder, or some other folder either above or below the currently active folder. The type of files (i.e., the extension) that can be saved is specified by assigning an appropriate string to the CommonDialog1.Filter property, as shown previously. The file type can be changed by clicking on the down-arrow at the end of the box labeled Save as type.

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When a spectrometer is operated at a resolution lower than the intrinsic features of an object view, the representation of these features in the spectrum is broader and weaker, but the integrated intensity is correct Consequently, the radiometric characterization of a FTIR-based spectroradiometer is a straightforward operation that leads to a radiometrically sound calibration This is due to the intrinsically large number of spectral channels in FTIR-based systems In fact, the spectral response of a channel is given by the ILS function described in the previous section An FT-based spectroradiometer has a large number of overlapping and smoothly varying channels..

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A new device based on laser-excited fluorescence provides unparalleled detection of biological materials for which only minuscule samples may be available. This device, invented at the Ames Laboratories, received a 1991 R&D 100 award. The Ames Microfluor detector was developed to meet a need for an improved detection technique, driven by important new studies of the human genome, abuse substances, toxins, DNA adduct formation, and amino acids, all of which may be available only in minute amounts. Although powerful and efficient methods have been developed for separating biological mixtures in small volumes (i.e., capillary electrophoresis), equally powerful techniques for subsequent detection and identification of these mixtures have been lacking. The Microfluor detector combines very high sensitivities with the ability to analyze very small volumes. The instrument design is based on the principle that many important biomaterials are fluorescent, while many other biomaterials, such as peptides and oligonucleotides, can be made to fluoresce by adding a fluorescent tag. When a sample-filled capillary tube is inserted into the Microfluor detector and is irradiated by a laser beam, the sample will fluoresce. The detector detects, monitors, and quantifies the contents by sensing the intensity of the fluorescent light emitted. The signal is proportional to the concentration of the materials. The proportionality constant is characteristic of the material itself. Analyses can be performed with sample sizes 50 times smaller than those required by other methods, and concentrations as low as 10 11 molar (1 part per trillion) can be measured. Often, the critical components in a sample are present at these minute concentrations. These two features make the Microfluor detector uniquely compatible with capillary electrophoresis. In addition, the Ames-developed detector is distinct from other laser-excited detectors in that it is not

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