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moment, the cyclosome ubiquitinates the inhibitor, causing it to break down and freeing the separin to break down cohesin. This liberates the sister chromatids from each other (and is the instant when chromatids become chromosomes). The spindle then separates the sister chromatids in two stages, called anaphase A and anaphase B. In anaphase A, the chromosomes move toward the poles ( g. 3.14). During this process, the kinetochore itself acts as a microtubule motor, disassembling microtubules as it moves down them, pulling the chromosomes along ( g. 3.15). Thus, metacentric chromosomes appear V-shaped (as in g. 3.15), subtelocentrics appear J-shaped, and telocentrics appear rod-shaped. In anaphase B, the spindle itself elongates as overlapping interpolar microtubules slide apart. The general elongation of the spindle pulls the chromosomes apart.

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where V and I are the peak amplitudes of the sinusoidal voltage and current, respectively, and V and I are their phase angles Two such waveforms are plotted in Figure 72, with unit amplitude and with phase angles V = /6 and I = /3 From here on, let us assume that the reference phase angle of the voltage source, V , is zero, and let I =

region of a metaphase chromosome from the plant Haemanthus katherinae. Spindle ber bundles on either side of the centromere extend in opposite directions. A ber not connected to the kinetochore is visible lying over the centromere. These bers are 60 to 70 nm in diameter. (Waheeb

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Voltage waveforms for unity amplitude, zero deg voltage phase angle and 60 deg current phase angle Voltage Current

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K. Heneen, The centromeric region in the scanning electron microscope, Hereditas, 97 (1982): 311 14. Reproduced by permission.)

04 02 0 02 04 06 08 1 0 001 002 003 004 005 006 007 008 009 Time (s) 01

Since the instantaneous power dissipated by a circuit element is given by the product of the instantaneous voltage and current, it is possible to obtain a general expression for the power dissipated by an AC circuit element: p(t) = v(t)i(t) = V I cos( t) cos( t ) (72)

At the end of anaphase ( g. 3.16), the separated sister chromatids (now full- edged chromosomes) have been pulled to opposite poles of the cell. The cell now reverses the steps of prophase to return to the interphase state ( g. 3.17).The chromosomes uncoil and begin to direct protein synthesis. A nuclear envelope re-forms around each set of chromosomes, nucleoli re-form, and cytokinesis takes place. The spindle breaks down into tubulin subunits; a residual of microtubules remains at

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Equation 72 can be further simpli ed with the aid of trigonometric identities to yield VI VI (73) cos( ) + cos(2 t ) 2 2 where is the difference in phase between voltage and current Equation 73 illustrates how the instantaneous power dissipated by an AC circuit element is equal to the sum of an average component, 1 V I cos( ), plus a sinusoidal component, 2 1 V I cos(2 t ), oscillating at a frequency double that of the original source 2 frequency The instantaneous and average power are plotted in Figure 73 for the signals of Figure 72 The average power corresponding to the voltage and current signals of equation 71 can be obtained by integrating the instantaneous power over one cycle of the sinusoidal signal Let T = 2 / represent one cycle of the sinusoidal signals Then the average power, Pav , is given by the integral of the instantaneous power, p(t), over one cycle: p(t) = Pav = 1 T

(b) Multi-recess circular pads Other than having a circular shape, hydrostatic pads can have shapes such as square, rectangular, annular recess circular, conical spherical, multi-recess circular, multi-recess rectangular, rectangular with radiussed recess corners and annular multi-recess Example: Multi-Recess Circular Pad Hydrostatic Bearings The design for the foregoing example is repeated for the multi-recess circular pad arrangement as shown in Figure 658 (a) The required design data are presented in Figure 658 (b) As the suggested design value for C/R is between 015 and 025, the C/R ratio for the design purpose of this problem is assumed to be 020 Therefore, from Figure 658 (b), the coefficients A and B are read as 066 and 22, respectively The bearing pad area is calculated as follows: F = A pR A p

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During metaphase, the chromosomes move to the equator of the cell. With the attachment of the spindle bers and the completion of the spindle itself, the chromosomes jockey into position in the equatorial plane of the spindle, called the metaphase plate. This happens as kinetochore microtubules exert opposing tension on the two sister kinetochores. Alignment of the chromosomes on this plate marks the end of metaphase ( g. 3.13).

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