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During anaphase, the sister chromatids separate and move toward opposite poles on the spindle.The physical separation of the sister chromatids and their movement to opposite poles are two separate activities. Chromatid separation represents a checkpoint in the process of mitosis; a surveillance mechanism will not allow the process to continue until all chromosomes are lined up on the metaphase plate with their sister kinetochores held by microtubules from opposite poles. The surveillance mechanism somehow checks the physical tension the spindle bers exert on a pair of sister chromatids; an unpaired chromatid can delay or stop the process. Initially, an inhibitory protein called securin binds an enzyme called separin that can break down cohesin, the complex holding the chromatids together. At the correct

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Figure 1433 (a) High-level block diagram of microcontroller; (b) Internal organization of microcontroller

How do we know when the phenotype is controlled by a plasmid rather than by the chromosomal genes of a bacterium Plasmids can be seen with an electron microscope or by density-gradient centrifugation of the cell s DNA. But several less direct lines of evidence also supply the answer. To begin with, multiple aspects of the phenotype (e.g., resistance to several antibacterial agents) change simultaneously, as with plasmid R222. Another clue is that the phenotypic change is infectious: Japanese workers found that with R222, resistant cells converted nonresistant cells. As B. Lewin stated, Resistance is infectious. Several other clues point to the presence of a plasmid. In linkage studies, using transduction for example,

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Ap = 1515 in2 977515 mm2 The radius, R, can be determined from the following relation: Ap = pR2 R =

for the execution of each instruction step Each step takes one clock cycle to complete We are all familiar with the rating of processor speeds in megahertz Typical microcontrollers are capable of speeds in the megahertz to tens of megahertz range

The CPU needs to have access to different kinds of memory to execute programs Read-only memory (ROM) is used for permanent programs and data that are necessary, for example, to boot and initialize the system Information stored in ROM remains unchanged even when power to the computer is turned off Random

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plasmid loci show no linkage to host loci; plasmids themselves can be mapped because their loci are linked to each other. Since the plasmid DNA replicates at its own speed, it can miss being incorporated into a daughter cell.Thus, many spontaneous losses of the plasmid occur. And nally, certain treatments with acridine dyes, for example have little effect on the replication of the host chromosome, but selectively prevent the plasmid from replicating; thus the plasmid can be eliminated from the cell population. The existence of plasmids in a bacterial population can, therefore, be veri ed with morphological, physiological, and analytical evidence.

C = 020R C = 020 (220) C = 044 in 1118 mm The clearance, h, is recommended to be between 0001 and 001 in Assume h = 0005 in = 0127 mm The viscosity can be obtained from Figure 635 for a given type of lubricant and temperature In this case, it is assumed that viscosity, = 83 10 6 lbs/in2 The oil flow can be approximated as

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