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3. The maroon-like (ma-l) locus in Drosophila is inherited in an X-linked recessive fashion. If you cross a heterozygous female with a maroon-like male, all the progeny are wild-type. If the female progeny from this cross are mated again with maroon-like males, half of the females produce all maroon-like progeny, and the other half produce all wild-type progeny. Explain these results. (See also MATERNAL EFFECTS)
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*Answers to selected exercises and problems are on page A-20.
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resistance looking in at terminals a and b if terminals c and d are open and again if terminals c and d are shorted together Also, nd the equivalent resistance looking in at terminals c and d if terminals a and b are open and if terminals a and b are shorted together
Before lysis, when phage DNA is being packaged into phage heads, an occasional error occurs that causes bacterial DNA to be incorporated into the phage head instead. When this happens, bacterial genes can be transferred to another bacterium via the phage coat. This process, called transduction, has been of great use in mapping the bacterial chromosome.Transduction occurs in two patterns: specialized and generalized.
Fig 660: Dimensions of a rectangular pad [17]
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The process of specialized or restricted transduction was rst discovered in phage by Lederberg and his students. Specialized transduction is analogous to sexduction it depends upon a mistake made during a loopingout process. In sexduction, the error is in the F factor. In specialized transduction, the error is in the prophage. Figure 7.25 shows the prophage looping out incorrectly to create a defective phage carrying the adjacent gal locus. Since only loci adjacent to the phage attachment site can be transduced in this process, specialized transduction has not proven very useful for mapping the host chromosome.
540
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The McGraw Hill Companies, 2001
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Dynamic viscosity, h = 5 10 6 lbf s in 2 0345 N s m 2 Supply pressure, Ps = 225 lbf in 2 155 106 N m 2 1 (maximum) Relative sliding velocity, n = 50 in s 127 m s 1 Number of pads, n = 10 A condition when the applied load is equal to the design load and the clearance, h, is equal to the design clearance, hd, is known as the design condition In addition, if r(int) = 0414c, the design can be greatly simplified Furthermore, the value of the resistance ratio, z, is taken as unity because this value gives the highest stiffness for any design thrust capacity Inflow Resistance, Ri capillary inflow restrictor For an inflow restrictor of a simple capillary tube of a constant and circular cross-section, assuming that the flow occurs in the capillary is purely laminar, and the drop in pressure locally at the inlet to and exit from the capillary is negligible, the inflow resistance, Ri, is given by 128l Ri = 4 dR Ri =
2
Generalized transduction, which Zinder and Lederberg discovered, was the rst mode of transduction discovered. The bacterium was Salmonella typhimurium and the phage was P22. Virtually any locus can be transduced by generalized transduction. The mechanism, therefore, does not depend on a faulty excision, but rather on the random inclusion of a piece of the host chromosome within the phage protein coat. A defective phage, one that carries bacterial DNA rather than phage DNA, is called a transducing particle. Transduction is complete when the genetic material from the transducing particle is injected into a new host and enters the new host s chromosome by recombination. For P22, the rate of transduction is about once for every 105 infecting phages. Since a transducing phage can carry only 2 to 2.5% of the host chromosome, only genes very close to each other can be transduced together (cotransduced). Cotransduction can thus help to ll in the details of gene order over short distances after interrupted mating or transformation is used to ascertain the general pattern. Transduction is similar to transformation in that cotransduction, like co-occurrence in transformation, is a relative indicator of map distance.
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